Journal: BMC Immunology

Article Title: Phenotypic and functional stability of leukocytes from human peripheral blood samples: considerations for the design of immunological studies

doi: 10.1186/s12865-019-0286-z

Figure Lengend Snippet: Chemokine and cytokine gene expression in PBMCs. Gene expression of CXCL3 , CCL2 , CCR7 , IL-1ß , TNFα , CCL8 , CCR2 and CXCL10 ( a - h ), was measured by qRT-PCR. Data are presented as mean and statistical significance estimated by one-way ANOVA followed by Dunnett’s multiple comparison test. *: p < 0.05, **: p < 0.01, ***: p < 0.001

Article Snippet: Gene expression of inflammatory mediators was evaluated using Taqman probes (Thermofisher Scientific) for CCL8 (Hs00271615_m1), CCL2 (Hs00234140_m1), CXCL3 (Hs00171061_m1), CXCL10 (Hs01124251_g1), CCR2 (Hs00704702_s1), CCR7 (Hs01013469_m1), TNF α (Hs00174128_m1), IL-1ß (Hs01555410_m1) and GAPDH (HS99999905_m1), in a BioRad® CFX-96 detection platform.

Techniques: Gene Expression, Quantitative RT-PCR, Comparison

Journal: Oncology Letters

Article Title: CMTM4 promotes the motility of colon cancer cells under radiation and is associated with an unfavorable neoadjuvant chemoradiotherapy response and patient survival in rectal cancer

doi: 10.3892/ol.2025.14884

Figure Lengend Snippet: CXCL8 was negatively associated with CMTM4 and indicated an inferior outcome in patients with LARC. (A) RT-qPCR of RNA-seq-identified CMTM4 targets in the shCM4-3 and shCM4-6 cells, compared with NC cells. The level of GAPDH transcripts was used for normalization. (B) RT-qPCR analysis of RNA-seq-identified CMTM4 targets in the CMTM4 overexpression cells, compared with the NC cells. The level of GAPDH transcripts was used for normalization. (C) CXCL8 expression in the intratumor region was negatively correlated with CMTM4 expression in patients with LARC (n=56; P=0.012). (D) CXCL8 expression in the invasive margin region was negatively associated with CMTM4 expression in patients with LARC (n=56; P=0.049). (E) K-M analysis indicated that CXCL8 expression in tumor margins was correlated with a shorter DFS (95% CI, 1.189–6.166; P=0.018). (F) K-M analysis indicated that CXCL8 expression in tumor margins was not associated with OS in patients with LARC (P=0.383). *P<0.05, **P<0.01 and ***P<0.001. NC, negative control. CXCL8, chemokine (CXC motif) ligand 8; CMTM4, CKLF-like MARVEL transmembrane domain member 4; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; K-M, Kaplan-Meier; CI, confidence interval; DFS, disease-free survival; OS, overall survival; OE, overexpression; sh, short hairpin.

Article Snippet: The sections were incubated with the following primary antibodies at 4°C overnight: chemokine (CXC motif) ligand 8 (CXCL8; 1:500; cat. no. 27095-1-AP; Proteintech Group, Inc.) and CMTM4 (1:500; cat. no. HPA014704; Sigma-Aldrich; Merck KGaA).

Techniques: Quantitative RT-PCR, RNA Sequencing, Over Expression, Expressing, Negative Control, Reverse Transcription, Real-time Polymerase Chain Reaction